C# .NET med SQL - Fejl imellem side og database

Hej... Jeg er lige begyndt at rode med noget C# .Net. Jeg har det problem, at jeg hverken kan læse fra / skrive til min database. Hvis jeg vil læse fra databasen får jeg denne fejlmeddelelse: Login failed for user 'f33933'. Reason: Not associated with a trusted SQL Server connection.

Jeg bruger dette til at connecte til serveren:

<add key="SQLcon" value="data source=DKDN01XX24;initial catalog=Jakob;password=********;persist security info=True;user id=f33933;workstation id=w74607;packet size=4096" />

Jeg har 2 buttons som skal hente og skrive til databasen.

Min kode:

using System;
using System.Collections;
using System.ComponentModel;
using System.Data;
using System.Data.SqlClient;
using System.Drawing;
using System.Web;
using System.Web.SessionState;
using System.Web.UI;
using System.Web.UI.WebControls;
using System.Web.UI.HtmlControls;
using System.Configuration;
using System.Data.SqlTypes;

namespace projekt
{
    /// <summary>
    /// Summary description for WebForm1.
    /// </summary>
    public class WebForm1 : System.Web.UI.Page
    {
        protected System.Web.UI.WebControls.TextBox TextBox3;
        protected System.Web.UI.WebControls.TextBox TextBox4;
        protected System.Web.UI.WebControls.Label Label1;
        protected System.Web.UI.WebControls.Label Label2;
        protected System.Web.UI.WebControls.Label Label3;
        protected System.Web.UI.WebControls.Label Label4;
        protected System.Web.UI.WebControls.Label Label5;
        protected System.Web.UI.WebControls.Label testLabel;
        protected System.Web.UI.WebControls.Label Labeltest;
        protected System.Web.UI.WebControls.Button Button2;
        protected System.Web.UI.WebControls.TextBox output;
        protected System.Web.UI.WebControls.TextBox Input;
        protected System.Web.UI.WebControls.ListBox ListBox1;
        protected System.Web.UI.WebControls.Button Button1;
       
        private void Page_Load(object sender, System.EventArgs e)
        {
            // Put user code to initialize the page here
        }

        #region Web Form Designer generated code
        override protected void OnInit(EventArgs e)
        {
            //
            // CODEGEN: This call is required by the ASP.NET Web Form Designer.
            //
            InitializeComponent();
            base.OnInit(e);
        }
       
        /// <summary>
        /// Required method for Designer support - do not modify
        /// the contents of this method with the code editor.
        /// </summary>
        private void InitializeComponent()
        {   
            this.Input.TextChanged += new System.EventHandler(this.TextBox1_TextChanged);
            this.Button2.Click += new System.EventHandler(this.Button2_Click);
            this.output.TextChanged += new System.EventHandler(this.TextBox2_TextChanged);
            this.Button1.Click += new System.EventHandler(this.Button1_Click);
            this.Load += new System.EventHandler(this.Page_Load);

        }
        #endregion

        private void Button1_Click(object sender, System.EventArgs e)
        {
            string barcode;
            barcode = Input.Text.ToString();
           
            testLabel.Text = barcode;

           
            string SQLconStr = ConfigurationSettings.AppSettings["SQLcon"];
            SqlConnection DB_Connection = new SqlConnection(SQLconStr);

            SqlDataAdapter StepAdapter = new SqlDataAdapter("INSERT INTO Jakob (barcode) VALUES('"+ barcode +"')", DB_Connection);
           
        }

        private void TextBox1_TextChanged(object sender, System.EventArgs e)
        {
           
        }

        private void TextBox5_TextChanged(object sender, System.EventArgs e)
        {
       
        }

        private void TextBox2_TextChanged(object sender, System.EventArgs e)
        {
       
        }

        private void Button2_Click(object sender, System.EventArgs e)
        {
           
            string SQLconStr = ConfigurationSettings.AppSettings["SQLcon"];
            SqlConnection DB_Connection = new SqlConnection(SQLconStr);

            SqlDataAdapter StepAdapter = new SqlDataAdapter("SELECT * FROM Jakob ORDER BY filename asc", DB_Connection);
           
           
            DataSet FileList = new DataSet();
            StepAdapter.Fill(FileList, "FileListSet");
           
            ListBox1.DataSource = FileList;
            ListBox1.DataMember = "FileListSet";
            ListBox1.DataTextField = "barcode";
           
            ListBox1.DataBind();
       

        }
    }
}